The minor seeds of Euphorbia rothiana plant belonging to the family Euphorbiaceae have been extracted with petroleum ether (40-60oC). The seed oil obtained was evaluated for their physicochemical characteristics as per standards of American oil chemical society (AOCS) procedures and analyzed by IR, GLC, NMR. The oil contains the saturated and unsaturated fatty acid viz. major quanties of linolenic rich (60.2%). The seed oil is nontoxic which exhibited significant anti-inflammatory (55.94%) and analgesic (66.20%) activities as compared with control and standard drug aspirin (91.10%) respectively.