Mukia maderaspatana (L.) M. Romer., a plant drug of Siddha medicine, is an annual monoecious tendril climber, belonging to the family Cucurbitaceae, mostly prevalent in South India. It is commonly called Musumusukkai in Tamil. The leaf and root part of the plant were collected and investigated for their physico-chemical properties. The leaf and root showed distinct variations. High performance thin layer chromatography analysis of ethyl alcohol extract of leaf and root samples gave three and five bands respectively. Both the leaf and root samples shows the Rf value 0.20 (β-sitosterol), the root sample shows the Rf value 0.56 (columbin).