Industrial pollution has been and continues to be a major threat to the environment affecting the water we use, the air we breathe and the soil we live on. Among the various industries, sugar mill is one of the largest and most important agro based industries in India. In this study, the content of heavy metals in the sugar mill effluent samples were analysed quantitatively. The presence of heavy metals like arsenic, cadmium, copper, lead, mercury, zinc, chromium was detected in all the samples analyzed.