The microorganisms present in water cause harmful effects in human body. The presence of bacteria and pathogenic (disease-causing) organisms can prove fatal and hence is a matter of great concern when considering the safety of drinking water. The pathogenic organisms can cause intestinal infections, dysentery, hepatitis, typhoid fever, cholera, and other illnesses. The paper discusses results of physicochemical and bacteriological analysis done on various water samples taken from different sources in Ahmedabad, Gujarat, India. The water samples collected from different regions of Ahmedabad were found to have significant impurities, considerable deterioration and remarkable variation. The samples were first collected and then stored in water storages made up of different materials like earthen material, plastic container. The study was conducted to analyse the change in basic properties of water samples over a significant period of time under varied environmental conditions made up of different materials. Various environmental conditions like under direct sun light or in room temperature which shows sunlight causes the elimination of microbes from water due to photo-degradation process. The tests were conducted and their results were studied on various physicochemical parameters such as pH, Total Dissolved Solids (TDS), Biological Oxygen Demand (BOD), Chemical Oxygen Demand (COD), and Dissolved Oxygen (DO).