Many herbs are usually practiced by Indonesian people to treat tooth pain, one of them is the roots of S. rhombifolia. However there are lack studies in dentistry to evaluate them either pharmacological activities. The aim of the present study was to investigate anti-inflammation and analgesic activities ethanol extract of S. rhombifolia roots. Anti-inflammatory using carrageenan-induced acute inflammation on rat and analgesic activities using hot plate method on mice, in vivo. Highest anti-inflammatory activities was found at dose 2.4 g/kg/BW with 20.78% of anti-inflammatory index compared to negative control (p<0.05). While the longest duration was for analgesic activities was found at concentration 2.4 g/kg/BW in 13.02 min. These results suggest that ethanol extract of S. rhombifolia roots have potential to be developed as dentistry formula.