Viruses that infect and accumulate in potatoes duri ng the planting process cause the low productivity and low quality of potatoes. The rapid stem tip culture wit h good quality can earn time and efficiency for the production of detoxified plantlets and potatoes. Therefore, it is necessary to optimize the detoxification culture m ethod of potatoes’ stem tips. We carried out experiments on the buds of Burbank’s pre-basic, basic, level 1 and commodity potatoes and on the test-tube plantlets by placing stripped stem tips on MS culture media with hormone in different densities, stripping the growing points on the stem tips and observing in the culture room. We calcula ted the pollution rate, survival rate and seeding rate and had ELISA virus detection on the acquired detoxifie d plantlets. The results showed that the growing points of all k inds of Burbank’s potatoes’ stem tips on MS+6-BA0.5mg/L+NAA0.01mg/L+GA30.1mg/L culture mediu m had higher survival rate and seeding rate (both above 80%) and detoxification rate (over 90%)than o thers. Compared with the control groups, the densit y of hormone in this culture medium was more conducive t o the stripping and seeding of all kinds of Burbank ’s potatoes’ stem tips.