Response surface methodology (RSM) was applied to predict optimum conditions for microwave-assisted extraction of total flavonoids extraction from Coreopsis tinctoria . A central composite design was used to monitor the effect of ratio of water to material, extraction time, microwave power on yield of total flavanoid. the optimal extraction conditions were obtained as ration of water to material of 70 mL/g, extraction time of 8 min and microwave power 590 W. under this conditions, the average total flavonoids yield was of 9.95% which matched with the predicted value of 9.99%. The extraction method was applied successfully to extract total flavonoids from Coreopsis tinctoria.