The objective of this study was to investigate effect of four emulsifiers including Span 20, Span 40, Span 60 and Span 80 on emulsifying effectiveness of phytosterol in milk through single factor and orthogonal experimental design to get the best emulsification conditions. The concentrations of four emulsifiers were all 0.2%, 0.3%, 0.4%, 0.5%, 0.6% and 0.7%. The optimal emulsified conditions showed as follows: the optimal concentrations of Span 20, Span 40, Span 60 and Span 80 were 0.35%, 0.50%, 0.60% and 0.45%, respectively, the emulsification R reached 1.022±0.0015 under the conditions mentioned above.