This paper reports a novel simultaneous uv-vis spectrophotometric determination of Bi (11I), Sn (

) Mn (II),V (III) and Se (II) in mixed aqueous solution using 3,3′,4′ ,5,7 – pentahydroxy flavuylium (cyanidin) as complexing agent. The maximum absorption wavelengths (λ) of Bi – cyanidin; Sn – cyanidin; Mn –cyanidin, V – cyanidin and Se – cyanidin complexes were 277 nm, 232 nm 240 nm, 272 nm and 251 nm respectively as against that of pure cyanidin which was 280 nm at the determined optimal pH value of 5. Spectral overlap did not occur among the metal complexes and linearity occurred with the range of 0.50 ppm to 2.5 ppm. This method has potentials for a simple, cheap, rapid and eco-friendly way of trace heavy metals determinations in biological and environmental samples.