Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2015 Vol: 7 Issue: 10

Non-toxic antiproliferative effect of Ficus carica fruit extracts on estrogen receptor positive breast cancer cell (MCF-7)

Abstract

Cancer is one of many diseases of global concern du e to its high mortality rate with various therapeut ic options but most of them are toxic and this compelled many canc er patients to seek alternative and complementary m ethod for treatment. Plants have been recognised as alternati ve source of remedy for various ailments as they co ntain many bioactive compounds. The objective of this study wa s therefore to determine the antiproliferative acti vity and non- toxic effect of dried preserved fruit extracts of F icus carica on estrogen/progesterone receptor posit ive breast cancer cell (MCF-7) and mouse epithelial cell (3T3) . The ethyl acetate, ethanol and hexane extracts we re prepared by maceration and then concentrated under vacuum at 40 o C by rotary evaporator to get the crude extracts. T he antiproliferative effect of the extracts was determ ined using microtitration colorimetric method of 3( 4, 5 diphenylmethyl thiazol-2-yl)-2, 5 diphenyltetrazoli um bromide assay. The ethyl acetate has low extract yield compared to ethanol and hexane extracts. The ethyl acetate extract was found to have strong and signif icant antiproliferative activity against estrogen/progest erone receptor positive breast cancer cells (MCF-7) with IC 50 value of 9.8 μ g/mL, whereas the ethanol and hexane extracts showe d weak antiproliferative activity. Interestingly, a ll the extracts have non-toxic effect on 3T3 cell line . These finding suggest that crude extracts of Ficu s carica fruit have antiproliferative effect on the estrogen/proge sterone receptor positive breast cancer cells. Thes e may be attributed to the bioactive compound present in the crude extracts. The study also suggests a promisin g chemoprotective potential of Ficus carica fruit ext racts on estrogen/progesterone receptor positive br east cancer cells. Further studies are required to isolate the bioactive compounds of the fruit extracts and estab lish their mechanism of actions