The aim of present investigation was to formulate and evaluate Niosomes, a synthetic microscopic vesicles consisting of an aqueous concentration is enclosed in a bilayer consisting of cholesterol and nonionic surfactants to improve the low corneal permeability for effective management trigeminal neuralgia. Proniosomal gels of flupirtine Maleate (centrally acting, non-opioid analgesic) were developed with span 20, span 60, span 80, tween 20 and tween 80 with cholesterol. Nonionic surfactant vesicles formed immediately upon hyadrating proniosomal gel. The morphological characteristics, entrapment efficiency, In-vitro drug release, drug release kinetic, stability studies, ocular irritation test, In vivo studies (eye wiping test) for Trigeminal neuralgia was determined. The entrapment efficiency (EE %) of flupirtine maleate was determined by centrifugation of freeze thawed vesicles followed the order span80 >span 60> span 20> tween 20> tween 80. The In vitro drug release studies showed that there was a prolong release of drug which followed Higuchi model. Niosome formed from span 80 and cholesterol is promising approach to prolong antinociception activity and improve permeation rate as compared to pure drug.