The mycological production of citric acid by Aspergillus niger NCIM-683 has been carried out in the presence of 3-acetamidocoumarin. The effect of coumarin compound on mycological production of citric acid has been observed and it was found that coumarin compound enhances the yield of citric acid to an extent of 7.793% higher in comparison to control fermentor flasks i.e. 6.880 g/100ml in 8 days of optimum incubation period, 1.8 pH and 30 0C temperature with 20% (w/v) molasses solution.