A series of new Schiff’s bases of Sulfanilamide 1a-8a were synthesized by condensation of sulfanilamide with different substituted aromatic aldehydes . The structures of these products were confirmed by physical and spectral analysis. Antibacterial and antifungal activity of the synthesized derivatives 1a-8a were done in comparison with Ciprofloxacin and Ketoconazole as standard compounds. All the 3 selected strains of bacteria and fungi namely S. Aureus, E. Coli and C. Albicans. Compounds 5a, 7a showed very good activity against C .Albicans while compounds 2a, 8a showed moderate activity when compared with standard drug Ketoconazole.