Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

header
Reach Us reach to JOCPR whatsapp-JOCPR +44 1625708989
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Original Articles: 2009 Vol: 1 Issue: 1

Method development and validation of RP- HPLC in the application of invitro dissolution study of Lamivudine in bulk drug and tablet formulation

Abstract

A new RP-HPLC method was developed for the determination of Lamivudine in the bulk drug and tablet dosage forms and it was applied for the in-vitro drug dissolution studies. Isocratic elution mode with a mixture of methanol and water in the ratio of (89:11) was selected as the mobile phase with a C18 column (250 x 4.6mm, 5μ) for separation. This mixture was found to be appropriate allowing good elution for the Lamivudine at retention time 2.72 minute at flow rate of 1 ml/min and detection wavelength at 272nm. The linearity was found in the concentration range of Lamivudine 5-100 μg/ml. The liquid chromatography method was extensively validated for linearity, range, accuracy, precision (intermediate precision, repeatability) and specificity. All these analytical validation parameters were observed and the %RSD was determined which indicates the useful of RP-HPLC method for determination of Lamivudine in the bulk drug & tablets dosage form.