Hydrazone are an important class of compounds found in many synthetic and pharmaceutical products. Due to their importance in synthetic and pharmaceutical chemistry, the present research paper reports Synthesis of Glyoxalbisisonicotinoylhydrazone( GINH ), Melting point, Elemental analysis, Spectral study & Antimicrobial activity are studied. A simple, sensitive & specific spectrophotometric method for the determination of Zn(II) is developed based on the colour reaction between zinc (II) and glyoxalbisisonicotinoylhydrazone ( GINH ). 1H NMR< Effect of diverse ion have been studied respectively. Stability constant of the complex, Dissociation constant & Change in free energy are determined. Composition of the metal & ligand has been determined by Job’s variation and mole ratio method. The optimum condition for complete colour development have been established by studying parameters like effect of medium, reagent concentration, time period have been studied.