Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2011 Vol: 3 Issue: 6

Macroporous Poly(Glycidyl Methacrylate-co-Divinylbenzene) Polymer Particles for the Immobilization of ?Ž?²-galactosidase from Aspergillus Oryzae

Abstract

Macroporous polymer particles containing surface epoxy groups were synthesized using glycidyl methacrylate(GMA) as monomer, divinylbenzene(DVB) as crosslinking agent, and dodecyl alcohol and cyclohexanol as porogenic agents by suspension copolymerization for immobilization of β-galactosidase.The Scanning electron microscopy (SEM) micrographs were done to characterize their surface structure. Under the optimum conditions, β-galactosidase was immobilized on the carrier obtained above, and satisfactory results were obtained in enzyme activity, pH stability, thermal stability, and operational stability. The conclusion obtained indicated that the ploy(GMA-co-DVB) was suitable to immobilize β-galactosidase from aspergillus oryzae.