Colorectal cancer (CRC) is a third most common of cancer in worldwide. A predictable mechanism for the development of CRC is an imbalance between cell renewal and cell death. Luteolin (LUT) is a bioflavonoid, has many beneficial effects such as antioxidant, anti-proliferative and anti-inflammatory. A novel approach is to develop a drug is to induce apoptosis. In this present study is to evaluate the apoptotic inducing property of LUT in Azoxymethane (AOM)-induced CRC in Balb/c mice. The levels of lysosomal enzymes were analyzed. Induction with AOM increased the levels of lysosomal enzymes and subsequent administration with LUT decreased the levels of lysosomal enzymes. Expression of Bax, Bcl2 and caspase3 were analyzed. LUT administration increases the expressions of Bax and caspase 3 and decreased the expression of Bcl2. At this juncture, LUT act as a strong chemotherapeutic agent by modulating lysosomal enzymes and inducing apoptosis.