Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2014 Vol: 6 Issue: 7

Low concentration of Cr(VI) inhibited proliferation and self-renewal of hepatic stem/progenitor cells by suppressing Wnt signaling activity

Abstract

The aim of the present study was to investigate the effect of low concentration of Cr(VI) on proliferation and self-renewal of hepatic stem/progenitor cells (HSPCs) and their possible mechanism of action. WB-F344 cells, a rat-derived multipotent HSPCs line, were cultured with various doses of Cr(VI) for 24 hours or 30 days. The results showed that low concentration of Cr(VI) inhibited proliferation and self-renewal of WB-F344 cells, in which cell colony formation efficiency and colony diameter were significantly decreased in a concentration dependent manner. Microarray and qPCR results revealed that the expression of several genes belonging to Wnt signaling were downregulated. Consistent with gene expression analysis, treatment of WB-F344 cells with Cr(VI) resulted in reduction of transcriptional activator b-catenin, together with its translocation from nucleus into cytoplasm. Our finding suggested that low concentration Cr(VI) inhibited proliferation and self-renewal activity of WB-F344 cells by suppressing Wnt signaling activity.