A simple, precise accurate and selective reversed phase high performance liquid chromatographic method for determination of triprolidine in preparations has been developed. In this method the active materials were separated using mobile phase consisting of ammonium acetate dissolved in 750 mL of deionized water and 4250 mL of absolute ethanol. The flow rate was 1.0 mL min-1 with UV detection at 232 nm. Chromatographic separation was achieved on ACE LC-Si (15.0 cm x 4.6mm, 5.0 μm) column. The method was found precise and linear over the concentration ranges of (0.057-17.058) mg/mL, with LOQ concentrations of (0.00208 mg/mL). Accuracy was found to be acceptable over levels 50 to 150 %. The results confirmed the selectivity of the analytical method.