Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2012 Vol: 4 Issue: 6

Keratin Degradation and Enzyme Producing Ability of Aspergillus Flavus and Fusarium Solani From Soil


Feather keratin is highly resistant to degradation, but some keratinase producing microorganisms can easily degrade these insoluble keratins. These keratinase producing species have an important application in removal of poultry waste and recycled into valuable by product. Aspergillus flavus and Fusarium solani was isolated from soil using wet mount technique and tested for degradation of keratin production of protease and lipase. Maximum percent degradation was noticed in F. solani of 33.6 ± 1.1 in 20 days at 28 ± 2oC. The production of different enzymes such as protease and lipase from A.flavus and F. solani. Maximum production of protease and lipase enzyme, were noted as A.flavus compared the F. solani. Protease and lipase enzyme have pH 7,6 and temperature 40oC, 55oC respectively. In the present study, sucrose content (70.6,61.3), Ammonium phosphate (73.3), Yeast extract (68.6) was noted in both protease and lipase enzyme.

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