The aim of present study was to isolate protease producing bacteria from soil samples collected from marine waste. The isolation was done by serial dilution and plating methods. All the isolates were screened for proteolytic activity on skim milk agar plate. A total of 7 from 18 bacteria were screened based on the zone of proteolysis. The maximum proteolytic activity was evaluated by primary and secondary screening methods. The significant isolate was identified using standard identification parameters and selected for further studies.