Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2018 Vol: 10 Issue: 5

ISOLATION AND SCREENING OF ANTIMICROBIAL ACTINOMYCETES FROM THE SOIL SURROUNDING DIFFERENT MEDICINAL PLANTS OF SAURASHTRA WITH FUTURE SCOPE TO PRODUCE ANTIMICROBIAL COMPOUNDS THEREFROM.

Abstract

Background: The need for new and useful compounds to provide assistance and relief in all aspects of the human condition is ever growing. The screening approach has been employed extensively in the search for microorganisms capable of producing useful antibiotics. Actinomycetes have the capability to synthesize those substances. Objective: The object behind research is isolation and screening of Actinomycetes producing new useful antimicrobial compound which may cause death of microbes which has been got resistance. Methods: In our study we are selected 11 medicinal plants from 3 regions of saurashtra. Soil sample collected from microflora part up to 20 cm depth of soil, diluted up to 106. Different media used for isolation and make pure colony of actinomycetes. Colonies of actinomycets were selected then color of Aerial and vegetative mycelium was studied. Cultures of isolates were prepared on ISP media & morphological charecteristics studied. Results: Out of 66 isolates 58 were identified up to generic level. For primary screening for antimicrobial activity we used cross streak method. It was found that out of 66 isolates 45 (68.2 %) isolates were showing activity against test microbes and 21 (31.8 %) isolates not showing any activity. Conclusion: it was concluded that actinomycetes isolates from different soil samples around medicinal plant area of saurashtra region are good source to produce antimicrobial compounds. Too many ecological niches still remain unexplored, needs to be studied for novel actinomycetes.