Isolation and characterization of triterpenoid compounds from Hippobroma longiflora plants have been done. Hippobroma longiflora leaf powder was extracted by maceration method using hexane, ethyl acetate and methanol. Ethyl acetate fraction was columned by chromatography colomn using silica gel as a stationary phase and hexane, ethyl acetate, acetone and methanol as mobile phase. The isolation result is in the form of 22 mg white crystals and leave a single spot with some comparisons eluent on a thin layer chromatography. Isolated compound was characterized using UV spectroscopy, FTIR and NMR.