Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2016 Vol: 8 Issue: 6

In-Vivo Genotoxicity and Cytotoxicity Study of Prazosin HCl

Abstract

The aim of performing this work is to study and evaluate Genotoxicity and Cytotoxicity of Prazosin HCL in mice. The animals were treated with Prazosin (PZ) at the doses of 5, 15, 25 mg/kg/body weight intraperitoneal (IP) for single dose (14 days) toxicity studies. Different methods were used to perform the study like Measurement of body weight, organ weight and food intake, Estimation of Malondialdehyde (MDA) level, Estimation of Reduced Glutathione (GSH) level, For evaluation of Genotoxicity following parameters has been evaluated: Micronucleus assay in bone marrow, Micronucleus assay in peripheral blood, Determination of DNA damage: Metaphase chromosome analysis, Determination of DNA damage: DNA fragmentation assay, Determination of Cytotoxicity: Histological examination.The results obtained clearly demonstrate that PZ produced toxic responses at the higher dose in the hepatocytes as evident from increased MDA level, decreased GSH level, DNA damage, increased DNA fragmentation in mice. Also, it is interesting that in bone marrow cells, PZ induced structural chromosomal aberrations, and significantly DNA strand breakage observed. So it is considered as Genotoxicity toward the bone marrow cells and to the hepatocytes of mice. The present study provided evidence that Prazosin induced significant genotoxic effects in mice at its equivalent hepatotoxic dose level.