Original Articles: 2011 Vol: 3 Issue: 1
In-vitro antioxidant activity of alcoholic leaf extract and subfractions of Alangium lamarckii Thwaites
Oxidative stress due to generation of reactive oxygen species and free radicals play a role in pathogenesis of various diseases, the aim of present study was to determine the antioxidant activity and total phenolic contents of alcoholic extract and its various sub-fractions of A. lamarckii (AL). Antioxidant activity of extract and sub-fraction were measured using a series of well-established assays including the 2,2-diphenyl-1-picrylhydrazyl (DPPH), the reducing power, the nitric oxide (NO.) and the hydrogen peroxide (H2O2) scavenging and hydroxyl radical assays. From all the fractions tested, ethyl acetate fraction had shown to possess the maximum scavengers of DPPH radical, nitric oxide, hydrogen peroxide and hydroxyl radical. The alcoholic extract exhibited a higher reducing power as compared to its overall sub-fraction. The total antioxidant capacity of the extract and sub-fractions were also comparatively evaluated and the antioxidant capacities were expressed as the number of equivalent of ascorbic acid. The strong antioxidant activities of AL may be attributed to its high level of polyphenolic compounds which was estimated spectrophotometrically using Folin-Ciocalteau assay method. The antioxidant property of the extract and sub-fractions of AL, as observed in the present study might be useful for the development of newer and more potent antioxidants.