The methanolic extract and Isolated pure compound of the leaves of Passiflora mollissima were screened against antibacterial activity with six different bacterial strains. Gram positive bacteria, viz., Staphylococcus aureus, Staphylococcus faecalis, Bacillus subtilis and Gram negative bacteria, Viz., E.coli, Proteus vulgaris, Salmonella typhi. by Disc Diffusion Technique in Nutrient agar medium. The effect produced by the extract was compared with the effect produced by the standard Ciprofloxacin 5μg/disc for bacteria.