Colon cancer (CRC) is one of the most common malignancies in many regions of the world. Luteolin, a 3′,4′,5,7- tetrahydroxyflavone, consist of many beneficial effects such as antioxidant, anti-proliferative, controls the glycoproteins in malignant and induce apoptosis during AOM-induced colon cancer. In this study, was aimed to analyze the effect of Luteolin on membrane bound ATPases in Azoxymethane (AOM)-induced colon cancer. Male Balb/C mice were divided into four groups: normal control, AOM-induced, AOM induced with Luteolin treated, normal control treated with Luteolin. CRC was induced by administration with AOM (15mg/kg body weight) intraperitoneally (i.p.) once in week for three weeks. The activities of Na+ K+-ATPase Ca2+-ATPase and Mg2+- ATPase were decreased in AOM-induced group of mice. Upon treatment with Luteolin the activities were increased significantly. In conclusion, Luteolin may be act as a potent chemotherapeutic agent against colon cancer.