The present study was carried out to evaluate influence of polysaccharides from angelica and astragalus（AAP）on H22 hepatocarcinoma mice. The built H22 hepatocarcinoma models mice were treated with AAP, astragalus membranaceus polysaccharide (AMP), angelica sinensis polysaccharide (ASP) (50 mg/kg bw/day) and 5-FU (25 mg/kg bw/day) for 10 days, once a day, 0.2 mL/per. The major criteria for evaluating their antitumor effects including tumor inhibition rate, serum ALT and AST, spleen index and thymus index of mice. The AAP showed significant suppression effect on the growth of H22 tumor cell and serum ALT and AST (P<0.01). AAP could increase thymus index and resist the tumor-induced splenomegaly. And that these functions of AAP were more effective than AMP and ASP. These results indicated that AAP had an obvious antitumor activity in vivo.