Red roselle is an ornamental and medicinal plant. The purpose of this study was to investigate the effect of photoperiod on the phytochemical content of red roselle. Red roselle cultivated on photoperiod variation of 8,9,10, 11 and 12 hours. Photoperiod influenced the phytochemical content of red roselle calix. Phytochemical content observed were water extract, fiber, ash, poliphenol, iron, omega-3, vitamin B1 , vitamin C, fats and protein. Ten hours of photoperiod provided optimum phytochemical content.