Original Articles: 2014 Vol: 6 Issue: 3
Influence of extracts from Rodgersia aesculifolia Batal on cytokines
Human monocytes in vitro culture were divided into lipopolysaccharide (LPS) group, different concentration of medicated groups and the blank control group. Each group were added the same amount of LPS except the control group. Collecting supermatant after 12 hours in cultural incubator with constant temperature and humidity, measuring the absorbance values of each solution by enzyme linked immunosorbentassay(ELISA)method. To compute cytokine concentration of each groups. Measuring enthalpy by microcalorimetry. Observing the effects of Rodgersia aesculifolia ingredients on 3 proinflammatory cytokines. Results showed several extracts of Rodgersia aesculifolia could inhibit interleukin-1β(IL-1β), interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α)(P<0.05)in different degree. The optimum final concentration is 60μg·mL-1 INTRODUCTION .