In Ayurveda the medicinal values of plants are well documented and revealed in the literature since ancient times. The expeditions for such medicinal plants are increasing day by day on account of man’s quest for finding out newer compounds to health benefit. The potential source of vascular plants is still not completely explored for the community utility. The screening of such plants for phytochemical compounds in order to evaluate pharmacological effect has become a random tool, very few vascular plants group with respect to antibacterial activity were studied. In search for alternative ways of infectious disease control; essential oil from oregano were used in the present study to check their antifungal/antibacterial properties against pathogenic bacteria,yeast and Fungi Imperfecta using standard disc diffusion method In vitro.