Amorphophallus paeoniifolius is indigenous medicinal plants cultivated most part of Asia-pacific region and used in the field of traditional medicine. Main aim of this study is to investigate presence of phytochemical such as alkaloids, phenol, glycosides, saponins, phytosterols, flavonoids, tannins, steroids, and terepenoids and antibacterial potential of various extract of A. paeoniifolius using human bacterial pathogens by agar-well diffusion assay. In antibacterial activity, among four extracts, ethyl acetate extract showed maximum activity for Bacillus subtilis - MTCC 121 (7.5 mm zone of inhibition) and Staphylococcus aureus - MTCC 737 (7 mm of zone of inhibition). Aqueous extract showed 2.75 mm zone of inhibition against Klebsilla pneumonia - MTCC 109. Ethanolic extracts showed the zone of inhibition of 3.75 mm for Staphylococcus aureus - MTCC 737 and methanolic extract showed 4 mm of inhibition for Pseudomonas aeruginosa - MTCC 424.Thus the above studies give basic idea about importance of bioactive compound from this medicinal plants and we can go for further purification and isolation of biologically active molecules from the Amorphophallus paeoniifolius.