Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2015 Vol: 7 Issue: 1

In vitro evaluation of antimicrobial, antioxidant and larvicidal activities from extracts of Zeyheria tuberculosa (Vell) Bur. (Bignoniaceae)

Abstract

This study aimed to investigate in vitro antimicrobial properties from extracts of Zeyheria tuberculosa, by antimicrobial susceptibility testing as well as testing their potential larvicide, compared to larvae of the mosquito Aedes aegypti, and investigate possible antioxidant properties of the synthetic method by DPPH (2,2-diphenyl-2- picryl-1-hidrazila). All plant material was extracted with EtOH, and the leaf extract of spent process liquid/liquid extraction and column chromatography (CC), the stem was subjected to the technique of Spray drying and the stem bark was obtained by maceration and drying in rotary evaporators. Samples that showed sensitivity in the Drilling Test in Agar were qualitatively evaluated by determining the Minimum Inhibitory Concentration (MIC). The results showed that the filtration CHCl3 was moderately active against strains of S. aureus, S. epidermidis and P. mirabilis, as it presented a MIC < 125 μg/mL for the three microorganisms. The crude extract of the stem presented active against strains of S. aureus and S. epidermidis with a MIC < 62.5 μg/mL and MIC < 31.2 μg/mL, respectively, and moderately active front P. mirabilis, with a MIC < 125 μg/mL. The samples evaluated against synthetic DPPH (2,2- diphenyl-1-picryl-hidrazila) showed weak activity or were inactive. In addition, the samples evaluated against larvae in 4th stage of Aedes aegypti were considered inactive, with mortality less than 25%.