Antifungal activity of five solvent extract viz., Petroleum ether , Benzene, chloroform, methanol and ethanol extract of seeds of Psoralea corylifolia tested at 0.5, 2.0 and 3.0% concentration against five Fusarium species viz., F. equiseti, F. graminearum, F. moniliforme, F. oxysporum, and F. solani was evaluated in vitro condition. Among five solvents tested, petroleum ether extract recorded a significant antifungal activity compared to other four solvent extract . The Fraction I, II and III of all the five solvent extracts of seeds of P corylifolia revealed that Fraction II which consists of three bands recorded significant antifungal activity against all the five Fusarium species tested at 250, 500 and 700ppm concentration.