Alcoholic whole plant extracts of Calotropis procera and Trigonella foenum graecum L. showed in vitro cytotoxicity against different human cancer cell lines such as lung, neuroblastima, liver and colon. Sulforhodamine B dye (SRB) assay was done for in vitro cytotoxicity test assay against five human cancer cell lines namely of lung (A-549), liver (Hep-2) colon (502713, HT- 29) and neuroblastima (IMR-32). Cannabis sativa and Trigonella foenum graecum L. showed more than 70% growth of in some cell lines 79% and 83% in Cannabis sativa and Trigonella foenum graecum L. respectively, against lung (A-549) cell line. In case of liver (Hep-2) cell lines, Cannabis sativa showed cytotoxic activity but Trigonella foenum graecum L extracts showed no activity. Colon 502713 cell lines were inhibited by the extracts of Trigonella foenum graecum L only. HT-29 liver human cancer line and IMR-32 neuroblastima cell lines showed cytotoxic inhibition by both plant extracts.