The need for investigating the protective effects of herbal extracts and compounds from natural sources has gained considerable attention among researchers. Current study was aimed at investigating the in vitro anti-microbial activity of methanolic leaf extracts of the medicinal plant Bacopa monnieri (Brahmi)and in silicoOuter Membrane Protein X (OMPX) inhibitory activity of its active components. The antimicrobial potential methanolic extract was tested by antibiotic susceptibility testing (AST) using Kirby Bauer disc diffusion method and compared to that of commercial antibiotics. Three different bacterial strains were used in the study: Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. OMPX was docked against active compounds of Bacopa monnieriand the docked complexes were analysed using PyMol molecular viewer. Bacopaside I gave highest docking score against OMPX followed by bacopaside II, bacopaside A, β-sitosterol, luteolin and apigenin.