Diabetes has emerged as a major healthcare problem in India. Traditional medicines are practiced worldwide for treatment of type II diabetes mellitus since ancient times. Herbal remedies typically are part of traditional and folk healing methods with long histories of use. WHO suggests plants house a vast potential that can be harnessed to control diabetes. Rauvolfia tetraphylla and Shorea robusta showed presence of biologically active compounds such as alkaloids, flavonoids, phenolics, tannin, glycosidase, reducing sugars and terpenoids. The antimicrobial properties of plants have been proven effective against selected human pathogens such as Staphylococcus aureus and Klebsiella pneumoniae. Antioxidant activities of plants were performed using DPPH free radical scavenging assay, for different concentration of plant extracts. R.tetraphylla has produced higher efficiency of about 53.22% than S.robusta. Plant methanolic leaf extracts of R.tetraphylla was subjected to Alpha-amylase inhibition assay and the results were 87.47% inhibition whereas S.robusta showed low inhibition potential of 44.93%. This reveals that R.tetraphylla was very effective and significant in treating diseases. This confirms that the plant might protect cells from oxidative damage, resulting in certain diseases. Further studies would reveal the novel compound responsible for anti-diabetic activity of the plant.