The in vitro study was carried out on Paulownia hybrid to examine the effect of different concentrations of heavy metals Pb, Cd and Ni in culture media on growth characters, photosynthesis pigments and accumulation of heavy metals occurred in shoots and roots, NPK absorption and total carbohydrates content. Culture medium supplemented with high concentration of Pb (100 ppm) led to the highest in vitro survival percentage (85%), rooting (100%) and longest both shootlets and roots, survival percentage of acclimatizaed shoots, longest adapted plants and highest number of leaves formed per adapted plant as well as the accumulation of Pb element which was in highest values in both shoots and roots, respectively. The highest number of shootlets formed per explant, numbers of leaves and roots per shootlet were in the highest values with high concentration of Ni (20ppm) treatment which also significantly increased chl.b, carotenoids, total chlorophyll contents as well as total carbohydrates content. Cd (5ppm) had pronounced effect on stem fresh, dry weights and leaves fresh weight of micropropagated plants. Addition of Pb at (25 ppm) to the culture media significantly increased chlorophyll a content to the highest value, highest content of N% and K% in both shoots and roots as well as P% shoots content. The highest accumulation of Cd element in both shoots and roots, highest contents of P% in roots were obtained as a result of cadmium supplement to culture medium at 20 ppm. High concentration of Ni (80 ppm) caused the highest accumulation of Ni element in both shoots and roots, respectively.