Impact of calcium and magnesium in diet to determine offspring gender in high-sugar rabbit has been investigated. Diabetes mellitus is a chronic, widely spread disease in living species. Sex determination has scientific basis for prevention of genetic diseases in addition to social backgrounds.21 rabbits (obtained from veterinary college Bangalore), were divided in to three groups in the rabbits male to female 1:1. The first group was made diabetic with Ca and Mg, the second group was non diabetic with Ca and Mg, group third was control unit (Non-diabetic without Ca and Mg). It was found that the delivered offsprings female to male ratio were 3.4:1, 3.3:1 and 1.06:1 for the first, second and third groups respectively. Also, it was found that non diabetic rabbits fed with normal food yields maximum numbers of offspring (72), while non diabetic rabbits fed with Ca and Mg yields 56 and diabetic hamsters with Ca and Mg yield lowest numbers of offspring (49).