Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2011 Vol: 3 Issue: 5

Immunostimulatory effects of Cardiospermum halicacubum against Vibrio parahaemolyticus on tiger shrimp Penaeus monodon

Abstract

During the past 15 years, shrimp farming, mainly based on local species such as tiger shrimp Penaeus monodon, kuruma shrimp Marsupenaeus japonicas and the exotic species white shrimp Litopenaeus vannamei, have been particularly badly hit by epidemics associated with viruses and Vibrio bacteria like Vibrio alginolyticus and V. parahaemolyticus due to deteriorated environment. Shrimp lack an adaptive immune system and rely on innate immune responses against microbial invasion. Therefore, the health of shrimp and enhancement of its immunity are of primary concern. Immunostimulants are agents/factors that trigger the non-specific immune response and result in enhanced disease resistance. Several compounds have been reported to have immunostimulation properties. Many of these are derivatives or cellular components of bacterial, fungal or animal origin. Laminarin, barley, glucan, lactoferrin, levamisole, lipopolysaccharides, curdlan, scleroglucan, zymosan, inulin, chitosan, β-glucans, dextran, lentinan, krestin, saponins, herbal extracts, peptidoglycans and so forth, are some of the examples of immunostimulants used in shrimp/fish aquaculture. This present study was attempted to study the immunomostimulatory effects of plant extract from herbal plants on shrimp through fed diet. The plant extract was mixed with fed diet with different concentration and non-specific immune response and THC, Phenoloxidase, NBT and lysozyme activity were measured. After feed diet experiment shrimp were challenge with isolated Vibrio spp. pathogen and cumulative survival rate were calculated.

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