Proteases are the most important group of enzymes from a biotechnological perspective. They have a wide variety of applications in many fields. In this study, protease was isolated from Nicotiana tabacum, Moringa oleifera, Murraya koenigii and Coriandrum sativum .This extracted proteases were immobilized on calcium alginate beads and the specific activities of the immobilized enzyme were estimated and compared. It was found that immobilized protease activity was more in Nicotiana tabacum (4.40 units/mg of protein) followed by Moringa oleifera (2.46 units/mg of protein), Murraya koenigii (2.02 units/mg of protein) and was less in Coriandrum sativum (1.56 units/mg of protein). The optimum pH was also determined and was found to be 7.2 for all the species. The activity with respect to time of production of these samples were also analyzed. For Murraya koenigii the activity was high at 10th min and in case of Moringa oleifera and Nicotiana tabacum, it was at 15th min. Coriandrum sativum had highest activity at the 20th min.