Living cell uses glucose as an energy source and intermediary metabolite. Glucose is transported across the apical membrane of the enterocytes by SLC5A1 and later across their basal membrane by SLC2A2. Molecule movement by glucose transporter proteins works by facilitating diffusion. There are various kinds of materials were found to inhibit the uptake of glucose by the cell both in vivo and in vitro. The in vitro glucose uptake inhibition study was carried out in the blood cells of diabetic patients and liver cells of alloxan-induced diabetic mice using natural and artificial glucose uptake inhibitors. The present study included 5 diabetic patients in the age group of 54, 63, 65, 67 and 72 years and 5 alloxan-induced diabetic albino mice. All patients had glucose level greater than 150mg/dl. The patients were monitor during a period of one month. In the present study, the effect of various natural and synthetic compounds on glucose uptake by cells was studied and the results are reported in this paper.