In the study, Talaromyces purpurogenus T013, a filamentous fungi belonging to Talaromyces which was identified had been isolated from the high value Taxus baccata subsp. wallichiana. By phylogeny analysis, T. purpurogenus T013 showed the close to other T. purpurogenus and Penicillium prupurogeum. In order to find out a new source of antimicrobial and anticancer agents, T. purpurogenus cultured in YM was against five different kinds of pathogens including Salmonella typhi (16.77±0.21 mm), Pseudomonas aeruginosa (16.17±0.21 mm), Serratia marcescens (15.50±0.21 mm), Listeria monocytogenes (16.37±0.15 mm) and Vibrio parahaemolyticus (15.43±0.21 mm) by using the agar well diffusion method. There was cytotoxic ability on Hela cancer cell line at the concentration (100 mg/mL) by using sulforhodamin B assay. The chloroform fraction showed antimicrobial activities that suggested the purified agent with antimicrobial in this fraction will be obtained so far.