Mirabilis jalapa L., the Four-o'clock plant is a multibranched perennial herb of family Nyctaginaceae. The plant is rich in many biomolecules of pharmacological importance and has been used in traditional medicine. One of the secondary metabolites in this plant is Dopamine, a Catecholamine. In the present study, attempts were made for a reliable chromatographic fingerprint profile for dopamine and to quantify the same from flower, leaves, root and seed of four different cultivars of M.jalapa (white, pink, yellow and multicolour) by a simple, precise, accurate and rapid high-performance thin-layer chromatographic method. The seed of white flower cultivar showed a high content of dopamine compared to other parts of four cultivars (0.059%).