Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2010 Vol: 2 Issue: 3

HPLC method validation for simultaneous estimation of madecassoside, asiaticoside and asiatic acid in Centella asiatica


A simple, accurate, precise, faster reverse phase H PLC method has been developed and validated for simultaneous estimation of madecassos ide, asiaticoside and asiatic acid from extract and raw material of the herb Centella asiat ica (L.) Urban (Apiaceae). The linear range for madecassoside, asiaticoside and asiatic acid wa s 1026-32.06mcg/ml, 1016-31.75mcg/ml and 1024-32.32mcg/ml respectively. The retention time o f madecassoside, asiaticoside and asiatic acid was 10.836, 11.9036 and 18.4002 min respective ly and the recoveries of three analyte are 107.84%, 113.06% and 104.03% respectively. In the c urrent study the separation of three compounds has been completed within a period of ni neteen minute which save the precious time and as well as solvent consumption. The Proposed me thod can be used for the quality control of the raw material, extracts and assay of these three markers in Centella.

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