Moringa oleifera L. (Moringaceae) is a valuable medicinal plant with a high nutritional and industrial value. To analyse the major polyphenolic compounds rutin, quercetin and gallic acid present in the M. oleifera leaves, HPLC methods have been used. In the range of 10-30 μg for rutin, quercetin and gallic acid with determination coefficient (R2) good linearity was observed and linear regression y = 1232.9x + 106.05, y = 133.53x + 202 and y = 619.4x + 3442.5 were achieved in 0.98, 0.99 and 1.04 min with the retention time respectively. The LOQs for rutin, quercetin and gallic acid; 3.4, 10.6 and 4.4 μg/ml and LODs ≤4.0 μg/ml for all standards were achieved. The rutin (555.6±1.79 μg/g), quercetin (37.54±1.07μg/g) and gallic acid (48.5±1.34 μg/g) were recorded in M. olefera. Rutin was the most abundant flavonoid analysed in M. oleifera.