A new simple High Performance Thin Layer Chromatographic (HPTLC) method for determination of Cefixime and Ofloxacin in combined tablet dosage form has been developed and validated. The mobile phase selected was Methanol: Ethyl acetate: Ammonia (3.5: 3.5: 1.5 v/v/v) with UV detection at 295 nm. The retention factor for Cefixime and Ofloxacin were found to be 0.78 ± 0.10 and 0.61 ± 0.12. Results found to be linear in the concentration range of 50-500 ng/band for both Cefixime and Ofloxacin. The method has been successfully applied for the analysis of drugs in pharmaceutical formulation. The % assay (Mean ± S.D.) was found to be 99.89 % ± 0.14 for Cefixime and 102.2 % ± 0.11 for Ofloxacin. The method was validated with respect to linearity, accuracy, precision and robustness as per the International Conference on Harmonisation (ICH) guidelines.