A simple , rapid and accurate high performance liquid chromatography method is developed for determination of oxolamine citrates from active pharmaceutical ingredient. The assay of oxalamine citrate was achieved on Chromatopack Peer less basic C-18 (50mm x4.6 mm) 5 μ column. The mobile phase consists of buffer and acetonitrile (55:45 % v/v ). The buffer was, 0.01 M Ammonium Acetate. The detection was carried out at wavelength 230 nm. The method was validated for system suitability, linearity, accuracy, precision, robustness, stability of sample solution. The method has been successfully used to analyze oxolamine citrate from pharmaceutical formulation.