Original Articles: 2019 Vol: 11 Issue: 1
Hepatoprotective Effect of Spissum Extract from Immature Walnut based on Model of Paracetamol-Induced Acute Liver Injury in Rats
According to the World Health Organization (WHO), more than 2 billion people in the world suffer from hepatopathy. Medicine-induced liver injury presents 12.8 to 14.0% of all adverse reactions to medicinal products. Paracetamol (latin Paracetamolum) is one of the most commonly used analgesics, which is on the WHO Essential Medicines List. Paracetamol-induced liver toxicity is associated with accumulation of its toxic metabolite N-acetyl-p-benzoquinone imine (NAPQI) that is a free radical enhancing lipid peroxidation, disturbing the energy state and causing death of hepatocytes. Nowadays, the problem of effective therapy of liver disease has yet to be resolved and it determines the relevance of searching for or creating new effective remedies. Speaking from this perspective, the medicinal plant walnut (Juglans regia L.) attracts attention because it is a promising object for creation of new gastroprotective remedies with hepatoprotective action. The study of hepatoprotective action of SAAE 30 (spissum aqueous alcoholic extract) was conducted on the model of acute Paracetamol-induced liver injury (Paracetamol Darnitsa Tablets 500 mg No. 10). As a reference substance, the medicinal product Legalon 70 was used. The results of the study revealed that application of SAAE 30 optimized a potential increase in the activity of antioxidant liver protection, as evidenced by a 3.3 times increase in content of reduced glutathione (RG) with regard to animals with hepatitis. Growth of antioxidant protection affected by SAAE 30 provided inhibition of lipid peroxidation intensity, thus the content of TBA-reactants decreased by 1.7 times (p <0.05). The investigated extract showed a potential anticytolytic activity: ALT activity decreased significantly by 1.6 and 1.8 times, respectively. Under the influence of SAAE 30, glycogen content returned to normal and reached the same level as in intact animals. Application of SAAE 30 contributed to a 1.4 times reduction of cholesterol content in bile, along with 1.2 times increase of bile acids level and 1.4 times decrease of AP activity. As affected by SAAE 30, there was a 1.7 times increase of cholesterol-cholate ratio (CCR) with regard to the group of model pathology and a significant increase of bile secretion rate by 1.35. Conclusion: The stated results show that in the presence of paracetamol-induced toxic hepatitis, SAAE 30 contributes to an increase in antioxidant liver protection, normalizes the glycogen-synthetic, chole-excretion and cholate-forming abilities of the organ and has a positive effect on the regulation of lipid metabolism. Thus, under the conditions of oxidative stress of the hepatobiliary system, SAAE 30 shows a pronounced hepatoprotective effect, which is equated with the effect of silymarin-containing hepatoprotector Legalon 70.