The present study was taken up to evaluate the hepatoprotective activity of Averrhoa bilimbi fruit in wistar albino rats intoxicated using acetaminophen. The extract was administered at two different concentrations 250 mg/kg.b.wt and 500 mg/kg.b.wt as lower and higher dose respectively. Silymarin (100 mg/kg.b.wt/) was used as standard positive control. The liver marker enzymes serum gluatamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), Alkaline phosphatise (ALKP) were found to be two fold increased in the disease control group, and pre-treatment with A. bilimbi inhibited the increase of these enzymes in serum. The histopathology studies on the tissues of liver, kidney and heart showed safety and efficacy of the test extract. The present study revealed that A. bilimbi fruits have significant hepatoprotective activity.